The ConSurf Server
SERVER FOR THE IDENTIFICATION OF FUNCTIONAL REGIONS IN BIOPOLYMERS
Job title
Human Insulin (ConSeq)
Date
13/06/2022
Running Parameters
Structure
Not Given. Running in 'ConSeq' mode
Phylogenetic Progress
Neighbor Joining with ML distance
Conservation Scores
Method of Calculation: Bayesian
Model of substitution for proteins: Best fit
Alignment
Multiple Sequence Alignment is built using MAFFT
The Homologues are collected from UNIREF90
Homolog search algorithm: HMMER
HMMER E-value: 0.0001
No. of HMMER Iterations: 1
Maximal %ID Between Sequences : 95
Minimal %ID For Homologs : 35
150 sequences that sample the list of homologues to the query.
Run Progress
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Find sequence homologs
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Remove redundant and unrelated sequences
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Align sequences
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Select best evolutionary model
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Calculate conservation scores
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Search for 3D structure for the protein sequence
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Predict 3D structure using HHPred and MODELLER
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Project conservation scores onto the molecule
Running Messages
There are 390 HMMER hits. 336 of them are unique, including the query.
The calculation is performed on a sample of 150 sequences that represent the list of homologues to the query.
Here is the list of sequences that produced significant alignments, but were not chosen as hits.
The best evolutionary model was selected to be: JTT. (details).
Some processes are being moved to a low priority queue. The calculation may take a longer time.