The ConSurf Server

SERVER FOR THE IDENTIFICATION OF FUNCTIONAL REGIONS IN BIOPOLYMERS

The ConSurf Server

SERVER FOR THE IDENTIFICATION OF FUNCTIONAL REGIONS IN BIOPOLYMERS

ConSurf Job Status Page - FINISHED

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Job title


Human Insulin (ConSeq)

Date


13/06/2022

Running Parameters

Structure


Not Given. Running in 'ConSeq' mode

Phylogenetic Progress


Neighbor Joining with ML distance

Conservation Scores


Method of Calculation: Bayesian

Model of substitution for proteins: Best fit

Alignment


Multiple Sequence Alignment is built using MAFFT

The Homologues are collected from UNIREF90

Homolog search algorithm: HMMER

HMMER E-value: 0.0001

No. of HMMER Iterations: 1

Maximal %ID Between Sequences : 95

Minimal %ID For Homologs : 35

150 sequences that sample the list of homologues to the query.

Run Progress



Find sequence homologs
Remove redundant and unrelated sequences
Align sequences
Select best evolutionary model
Calculate conservation scores
Search for 3D structure for the protein sequence
Predict 3D structure using HHPred and MODELLER
Project conservation scores onto the molecule

Running Messages



There are 390 HMMER hits. 336 of them are unique, including the query.
The calculation is performed on a sample of 150 sequences that represent the list of homologues to the query.

Here is the list of sequences that produced significant alignments, but were not chosen as hits.

The best evolutionary model was selected to be: JTT. (details).


Some processes are being moved to a low priority queue. The calculation may take a longer time.