ConSurf Job Status Page - FINISHED

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Job number and title:

Job number 1496496299, titled: Human Insulin (ConSeq) - Gallery

Running Parameters:

Run progress:

• Find sequence homologs
• Align sequences
• Select best evolutionary model
• Calculate conservation scores
• Search for 3D structure for the protein sequence
• Project conservation scores onto the molecule

Running Messages:

• MODELLER key was not provided, ConSurf run continues without structure prediction for your query sequence

• There are 187 HMMER hits. 174 of them are unique, including the query.
  The calculation is performed on a sample of 150 sequences that represent the list of homologues to the query.

• Click here if you wish to view the list of sequences which produced significant alignments, but were not chosen as hits.

• The best evolutionary model was selected to be: JTT. See details here
  

ConSurf calculation is finished:

Final Results

• The query sequence colored according to the conservation scores (HTML)(PDF)
  

• Multiple Sequence Alignment Color-Coded by Conservation

• View MSA and phylogenetic tree using WASABI and run ConSurf on sub-tree

• Multiple Sequence Alignment Color-Coded by Conservation and the (neighbor-joining) ConSurf tree (Download Chimera; required)
  To display the file:

  1. Save the chimerax file in your computer
  2. Open chimera program
  3. Load (File->Open...) the chimerax file from your computer in chimera

• Amino Acid Conservation Scores, Confidence Intervals and Conservation Colors

• Download all ConSurf outputs in a click!
  

Sequence Data

• HMMER output (HMMER hits with E-values and pairwise alignments)
  

• Sequences Used (displayed in FASTA format, linked to sequence data-base)
  

Alignment

• Multiple Sequence Alignment
  

      Alignment details
      The average number of replacements between any two sequences in the alignment;
      A distance of 0.01 means that on average, the expected replacement for every 100 positions is 1.
      Average pairwise distance : 0.802216
      Lower bound : 1.01758e-07
      Upper bound : 1.95116

• Residue variety per position in the MSA (The table is best viewed with an editor that respects Comma-Separated Values)
  

Phylogenetic Tree

• Phylogenetic Tree in Newick format
  

• View Phylogenetic Tree
  

Project ConSurf results on known structures similar to your sequence

When looking for known structures that share high similarity with your sequence, we have found 4 relevant structures.
Press here to show the list.