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Job number and title:
Job number 1496496299, titled: Human Insulin (ConSeq) - GalleryRunning Parameters:
Structure
Not Given. Running in 'ConSeq' mode
Not Given. Running in 'ConSeq' mode
Alignment
Multiple Sequence Alignment was built using MAFFT
The Homologues were collected from UNIREF90
Homolog search algorithm: HMMER
HMMER E-value: 0.0001
No. of HMMER Iterations: 1
Maximal %ID Between Sequences : 95
Minimal %ID For Homologs : 35
150 sequences that sample the list of homologues to the query.
Multiple Sequence Alignment was built using MAFFT
The Homologues were collected from UNIREF90
Homolog search algorithm: HMMER
HMMER E-value: 0.0001
No. of HMMER Iterations: 1
Maximal %ID Between Sequences : 95
Minimal %ID For Homologs : 35
150 sequences that sample the list of homologues to the query.
Phylogenetic Tree
Neighbor Joining with ML distance
Neighbor Joining with ML distance
Conservation Scores
Method of Calculation: Bayesian
Model of substitution for proteins: Best fit
Method of Calculation: Bayesian
Model of substitution for proteins: Best fit
Run progress:
- Find sequence homologs
- Align sequences
- Select best evolutionary model
- Calculate conservation scores
- Search for 3D structure for the protein sequence
- Project conservation scores onto the molecule
Running Messages:
- MODELLER key was not provided, ConSurf run continues without structure prediction for your query sequence
- There are 187 HMMER hits. 174 of them are unique, including the query.
The calculation is performed on a sample of 150 sequences that represent the list of homologues to the query.
- Click here if you wish to view the list of sequences which produced significant alignments, but were not chosen as hits.
- The best evolutionary model was selected to be: JTT. See details here
ConSurf calculation is finished:
Final Results
- Multiple Sequence Alignment Color-Coded by Conservation and the (neighbor-joining) ConSurf tree (Download Chimera; required)
To display the file:
- Save the chimerax file in your computer
- Open chimera program
- Load (File->Open...) the chimerax file from your computer in chimera
- Download all ConSurf outputs in a click!
Sequence Data
- HMMER output (HMMER hits with E-values and pairwise alignments)
- Sequences Used (displayed in FASTA format, linked to sequence data-base)
Alignment
Alignment detailsThe average number of replacements between any two sequences in the alignment;
A distance of 0.01 means that on average, the expected replacement for every 100 positions is 1.
Average pairwise distance : 0.802216
Lower bound : 1.01758e-07
Upper bound : 1.95116
- Residue variety per position in the MSA (The table is best viewed with an editor that respects Comma-Separated Values)
Phylogenetic Tree
Project ConSurf results on known structures similar to your sequence
When looking for known structures that share high similarity with your sequence, we have found 4 relevant structures.Press here to show the list.
Press on the plus sign ('+') near each structure id to show the relevant links for that structure.
+ PDB:2KQP CHAIN: A |
Show Alignment |
Show PDB Details
PDB ID: 2KQP Chain: A
%Identity between query and PDB: 96.5116279069768
%Similarity between query and PDB: 96.5116279069768
PSI-blast E-Value: 2e-42
Length of alignment overlap with query: 86
Length of alignment overlap with PDB: 86
Descriptions: EA14090CFFEA77F0 86 NMR NA NA NA no Insulin [Homo sapiens]
Learn more about the protein on Proteopedia | PDB
+ PDB:1EFE CHAIN: A |
Show Alignment |
Show PDB Details
%Identity between query and PDB: 96.5116279069768
%Similarity between query and PDB: 96.5116279069768
PSI-blast E-Value: 2e-42
Length of alignment overlap with query: 86
Length of alignment overlap with PDB: 86
Descriptions: EA14090CFFEA77F0 86 NMR NA NA NA no Insulin
Learn more about the protein on Proteopedia | PDB
>1EFEA FF6402C114868997 60 NMR NA NA NA no Insulin
Length = 60
Score = 105 bits (263), Expect = 1e-23, Method: Compositional matrix adjust.
Identities = 56/86 (65%), Positives = 57/86 (66%), Gaps = 26/86 (30%)
Query: 25 FVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEG 84
FVNQHLCGSHLVEALYLVCGERGFFYTPKTRR D+
1EFEA: 1 FVNQHLCGSHLVEALYLVCGERGFFYTPKTRRYPGDV----------------------- 37
Query: 85 SLQKRGIVEQCCTSICSLYQLENYCN 110
KRGIVEQCCTSICSLYQLENYCN
1EFEA: 38 ---KRGIVEQCCTSICSLYQLENYCN 60
Length = 60
Score = 105 bits (263), Expect = 1e-23, Method: Compositional matrix adjust.
Identities = 56/86 (65%), Positives = 57/86 (66%), Gaps = 26/86 (30%)
Query: 25 FVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEG 84
FVNQHLCGSHLVEALYLVCGERGFFYTPKTRR D+
1EFEA: 1 FVNQHLCGSHLVEALYLVCGERGFFYTPKTRRYPGDV----------------------- 37
Query: 85 SLQKRGIVEQCCTSICSLYQLENYCN 110
KRGIVEQCCTSICSLYQLENYCN
1EFEA: 38 ---KRGIVEQCCTSICSLYQLENYCN 60
PDB ID: 1EFE Chain: A
%Identity between query and PDB: 65.1162790697674
%Similarity between query and PDB: 66.2790697674419
PSI-blast E-Value: 1e-23
Length of alignment overlap with query: 86
Length of alignment overlap with PDB: 60
Descriptions: FF6402C114868997 60 NMR NA NA NA no Insulin [Homo sapiens]
Learn more about the protein on Proteopedia | PDB
+ PDB:2LWZ CHAIN: A |
Show Alignment |
Show PDB Details
%Identity between query and PDB: 65.1162790697674
%Similarity between query and PDB: 66.2790697674419
PSI-blast E-Value: 1e-23
Length of alignment overlap with query: 86
Length of alignment overlap with PDB: 60
Descriptions: FF6402C114868997 60 NMR NA NA NA no Insulin
Learn more about the protein on Proteopedia | PDB
>2LWZA 02DC70B294CD6F38 57 NMR NA NA NA no Insulin
Length = 57
Score = 90.9 bits (224), Expect = 5e-19, Method: Compositional matrix adjust.
Identities = 52/86 (60%), Positives = 53/86 (61%), Gaps = 29/86 (33%)
Query: 25 FVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEG 84
FVNQHLCGS LVEALYLVCGERGFFYT T GGGP
2LWZA: 1 FVNQHLCGSDLVEALYLVCGERGFFYTDPT--------------GGGP------------ 34
Query: 85 SLQKRGIVEQCCTSICSLYQLENYCN 110
+RGIVEQCC SICSLYQLENYCN
2LWZA: 35 ---RRGIVEQCCHSICSLYQLENYCN 57
Length = 57
Score = 90.9 bits (224), Expect = 5e-19, Method: Compositional matrix adjust.
Identities = 52/86 (60%), Positives = 53/86 (61%), Gaps = 29/86 (33%)
Query: 25 FVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEG 84
FVNQHLCGS LVEALYLVCGERGFFYT T GGGP
2LWZA: 1 FVNQHLCGSDLVEALYLVCGERGFFYTDPT--------------GGGP------------ 34
Query: 85 SLQKRGIVEQCCTSICSLYQLENYCN 110
+RGIVEQCC SICSLYQLENYCN
2LWZA: 35 ---RRGIVEQCCHSICSLYQLENYCN 57
PDB ID: 2LWZ Chain: A
%Identity between query and PDB: 60.4651162790698
%Similarity between query and PDB: 61.6279069767442
PSI-blast E-Value: 5e-19
Length of alignment overlap with query: 86
Length of alignment overlap with PDB: 57
Descriptions: 02DC70B294CD6F38 57 NMR NA NA NA no Insulin [Homo sapiens]
Learn more about the protein on Proteopedia | PDB
+ PDB:2JZQ CHAIN: A |
Show Alignment |
Show PDB Details
%Identity between query and PDB: 60.4651162790698
%Similarity between query and PDB: 61.6279069767442
PSI-blast E-Value: 5e-19
Length of alignment overlap with query: 86
Length of alignment overlap with PDB: 57
Descriptions: 02DC70B294CD6F38 57 NMR NA NA NA no Insulin
Learn more about the protein on Proteopedia | PDB
>2JZQA 02DC70B294CD6F38 57 NMR NA NA NA no Insulin
Length = 57
Score = 90.9 bits (224), Expect = 5e-19, Method: Compositional matrix adjust.
Identities = 52/86 (60%), Positives = 53/86 (61%), Gaps = 29/86 (33%)
Query: 25 FVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEG 84
FVNQHLCGS LVEALYLVCGERGFFYT T GGGP
2JZQA: 1 FVNQHLCGSDLVEALYLVCGERGFFYTDPT--------------GGGP------------ 34
Query: 85 SLQKRGIVEQCCTSICSLYQLENYCN 110
+RGIVEQCC SICSLYQLENYCN
2JZQA: 35 ---RRGIVEQCCHSICSLYQLENYCN 57
Length = 57
Score = 90.9 bits (224), Expect = 5e-19, Method: Compositional matrix adjust.
Identities = 52/86 (60%), Positives = 53/86 (61%), Gaps = 29/86 (33%)
Query: 25 FVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEG 84
FVNQHLCGS LVEALYLVCGERGFFYT T GGGP
2JZQA: 1 FVNQHLCGSDLVEALYLVCGERGFFYTDPT--------------GGGP------------ 34
Query: 85 SLQKRGIVEQCCTSICSLYQLENYCN 110
+RGIVEQCC SICSLYQLENYCN
2JZQA: 35 ---RRGIVEQCCHSICSLYQLENYCN 57
PDB ID: 2JZQ Chain: A
%Identity between query and PDB: 60.4651162790698
%Similarity between query and PDB: 61.6279069767442
PSI-blast E-Value: 5e-19
Length of alignment overlap with query: 86
Length of alignment overlap with PDB: 57
Descriptions: 02DC70B294CD6F38 57 NMR NA NA NA no Insulin [Homo sapiens]
Learn more about the protein on Proteopedia | PDB
%Identity between query and PDB: 60.4651162790698
%Similarity between query and PDB: 61.6279069767442
PSI-blast E-Value: 5e-19
Length of alignment overlap with query: 86
Length of alignment overlap with PDB: 57
Descriptions: 02DC70B294CD6F38 57 NMR NA NA NA no Insulin
Learn more about the protein on Proteopedia | PDB